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High-content imaging and gene expression analysis to study cell–nanomaterial interactions: The effect of surface hydrophobicity

Paper ID Volume ID Publish Year Pages File Format Full-Text
5957 450 2014 10 PDF Available
Title
High-content imaging and gene expression analysis to study cell–nanomaterial interactions: The effect of surface hydrophobicity
Abstract

The effects of nanoparticle (NP)-related parameters on cellular interactions are currently uncertain as analysis is complicated by the combinatorial diversity arising from the array of size, shape and surface properties. Here, we present a validated multiparametric high-content imaging method, with the utility of this approach demonstrated by in-depth analysis of the role of hydrophobicity on the interaction of Au NPs with cultured cells. In this methodology, we evaluate cell viability, membrane damage, induction of reactive oxygen species, mitochondrial health, cell area, skewness and induction of autophagy. High-content cell cycle phase studies and in-depth gene expression studies then serve to elucidate the underlying mechanisms. The data reveal a clear influence of the degree of NP surface hydrophobicity with membrane damage and autophagy induction, which is stronger than the effect of surface charge, for charges ranging between −50 and +20 mV. All labeling experiments occur in the same format, and can be further supplemented with additional parameters providing a broadly accessible format for studying cell-NP interactions under highly reproducible conditions.

Keywords
High-content imaging; Nanotoxicology; Nanoparticles; Cytotoxicity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 37, December 2014, Pages 9941–9950
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us