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Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission

Paper ID Volume ID Publish Year Pages File Format Full-Text
5977 452 2013 12 PDF Available
Title
Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission
Abstract

Human follicular thyroid cancer cells were cultured in Space to investigate the impact of microgravity on 3D growth. For this purpose, we designed and constructed a cell container that can endure enhanced physical forces, is connected to fluid storage chambers, performs media changes and cell harvesting automatically and supports cell viability. The container consists of a cell suspension chamber, two reserve tanks for medium and fixative and a pump for fluid exchange. The selected materials proved durable, non-cytotoxic, and did not inactivate RNAlater. This container was operated automatically during the unmanned Shenzhou-8 Space mission. FTC-133 human follicular thyroid cancer cells were cultured in Space for 10 days. Culture medium was exchanged after 5 days in Space and the cells were fixed after 10 days. The experiment revealed a scaffold-free formation of extraordinary large three-dimensional aggregates by thyroid cancer cells with altered expression of EGF and CTGF genes under real microgravity.

Keywords
Biocompatibility; Bioreactor; Growth factors; Apoptosis; Polyetheretherketone; Thyroid cancer
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Spheroid formation of human thyroid cancer cells in an automated culturing system during the Shenzhou-8 Space mission
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 31, October 2013, Pages 7694–7705
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us