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Impact of macrophage deficiency and depletion on continuous glucose monitoring in vivo

Paper ID Volume ID Publish Year Pages File Format Full-Text
5990 453 2014 8 PDF Available
Title
Impact of macrophage deficiency and depletion on continuous glucose monitoring in vivo
Abstract

Although it is assumed that macrophages (MQ) have a major negative impact on continuous glucose monitoring (CGM), surprisingly there is no data in the literature to directly support or refute the role of MQ or related foreign body giant cells in the bio-fouling of glucose sensors in vivo. As such, we developed the hypothesis that MQ are key in controlling glucose sensor performance and CGM in vivo and MQ deficiencies or depletion would enhance CGM. To test this hypothesis we determined the presence/distribution of MQ at the sensor tissue interface over a 28-day time period using F4/80 antibody and immunohistochemical analysis. We also evaluated the impact of spontaneous MQ deficiency (op/op mice) and induced-transgenic MQ depletions (Diphtheria Toxin Receptor (DTR) mice) on sensor function and CGM utilizing our murine CGM system. The results of these studies demonstrated: 1) a time dependent increase in MQ accumulation (F4/80 positive cells) at the sensor tissue interface; and 2) MQ deficient mice and MQ depleted C57BL/6 mice demonstrated improved sensor performance (MARD) when compared to normal mice (C57BL/6). These studies directly demonstrate the importance of MQ in sensor function and CGM in vivo.

Keywords
Macrophages; Diabetes; Implantable glucose sensors; Continuous glucose monitoring
First Page Preview
Impact of macrophage deficiency and depletion on continuous glucose monitoring in vivo
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 6, February 2014, Pages 1789–1796
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering