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A tetracycline expression system in combination with Sox9 for cartilage tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
6000 453 2014 9 PDF Available
Title
A tetracycline expression system in combination with Sox9 for cartilage tissue engineering
Abstract

Cartilage tissue engineering using controllable transcriptional therapy together with synthetic biopolymer scaffolds shows higher potential for overcoming chondrocyte degradation and constructing artificial cartilages both in vivo and in vitro. Here, the potential regulating tetracycline expression (Tet-on) system was used to express Sox9 both in vivo and in vitro. Chondrocyte degradation was measured in vitro and overcome by Soxf9 expression. Experiments confirmed the feasibility of the combined use of Sox9 and Tet-on system in cartilage tissue engineering. Engineered poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) scaffolds were seeded with recombinant chondrocytes which were transfected with Tet-induced Sox9 expression; the scaffolds were implanted under the skin of 8-week-old rats. The experimental group was injected with Dox in the abdomen, while the control group was injected with normal saline. After 4 or 8 days of implantation in vivo, the newly formed pieces of articular chondrocytes were taken out and measured. Dox injection in vivo showed positive effect on recombinant chondrocytes, in which Sox9 expression was up-regulated by an inducible system with specific matrix proteins. The results demonstrate this controllable transcriptional therapy is a potential approach for tissue engineering.

Keywords
Tet-on system; Sox9; Primary chondrocytes; Tissue engineering
First Page Preview
A tetracycline expression system in combination with Sox9 for cartilage tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 6, February 2014, Pages 1898–1906
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering