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Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines

Paper ID Volume ID Publish Year Pages File Format Full-Text
6014 453 2014 12 PDF Available
Title
Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines
Abstract

Myo-Inositol (INO) is a biomolecule with crucial functions in many aspects. In this study, hyperbranched copolymers for gene delivery were synthesized based on inositol and low molecular weight polyethylenimine. The capacity of INO-PEIs to load plasmid DNA and their biocompatibility was demonstrated. A tumor target ligand, folic acid (FA), which was widely used for drug delivery systems, was subsequently conjugated to INO-PEIs and resulted in INO-PEI-FA copolymers. The polymers were then evaluated on their activity to mediate transgene expression in mammalian cell lines. As indicated, INO-PEIs were able to mediate efficient transgene expression, which was particularly noticeable in carcinoma cell line HeLa. INO-PEI-FA further improved the efficiency in HepG2. Distribution of INO-PEI-FA polymers in non-carcinoma NIH 3T3 and carcinoma HeLa cell lines was discussed.

Keywords
Inositol (INO); Folic acid (FA); Polyethylenimine (PEI); Hyperbranched molecules; Transfection; Transgene expression
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Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 6, February 2014, Pages 2039–2050
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us