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Nanovesicles engineered from ES cells for enhanced cell proliferation

Paper ID Volume ID Publish Year Pages File Format Full-Text
6040 455 2014 9 PDF Available
Title
Nanovesicles engineered from ES cells for enhanced cell proliferation
Abstract

Extracellular vesicles (Exosomes and microvesicles) have drawn wide attentions in both diagnostic and therapeutic applications, since they are considered to shuttle biological signals intercellularly. However, further research on exosomes is limited by their rarity and heterogeneity even after lengthy isolation processes. In particular, these limitations are challenging in therapeutic applications. To meet these demands, cell-derived nanovesicles that mimic exosomes were generated by extruding living embryonic stem cells through micro-filters. These nanovesicles have an enclosed lipid bilayer and contain cellular contents. The present study investigated the ability of these nanovesicles to improve proliferation by treating primary murine skin fibroblasts with the nanovesicles. The treated skin fibroblasts showed higher expression levels of mRNA, VEGF-α, protein levels of TGF-β collagen I, PCNA, and Ki-67, as well as enhanced cell proliferation rate and number, compared to non-treated cells. The results indicate that treatment with the nanovesicles could potentially contribute to recovery or wound healing process of tissues.

Keywords
Nanoparticles; Cell proliferation; ECM; Fibroblasts; Growth factor; Stem cell
First Page Preview
Nanovesicles engineered from ES cells for enhanced cell proliferation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 34, November 2014, Pages 9302–9310
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering