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Corneal reinforcement using an acellular dermal matrix for an analysis of biocompatibility, mechanical properties, and transparency

Paper ID Volume ID Publish Year Pages File Format Full-Text
605 52 2012 7 PDF Available
Title
Corneal reinforcement using an acellular dermal matrix for an analysis of biocompatibility, mechanical properties, and transparency
Abstract

The aim of this study was to analyze the viability of using an acellular dermal matrix (ADM) as a reinforcement material for peripheral corneal thinning disease. The complete removal of cell components was confirmed by hematoxylin and eosin (H&E) and 4′,6-diamidino-2-phenylindole (DAPI) staining. Transmission electron microscopy determined that the stromal structure was well preserved. Uniaxial tests revealed that the ADM had strong mechanical properties. After being implanted into rabbit cornea the ADM showed no sign of rejection and even achieved good transparency 24 weeks post-surgery. H&E staining demonstrated that keratocytes grew in the ADM and the ADM–cornea interface became blurry. Picrosirius red staining revealed great changes of collagen in the ADM. Uniaxial testing of the reinforced cornea showed better mechanical strength than the normal rabbit cornea, but this did not exhibit statistical significance. These results suggest that ADM is a worthy candidate for future exploration as a reinforcement material for peripheral corneal thinning problems.

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Keywords
Biocompatibility; Collagen; Cornea; Mechanical properties
First Page Preview
Corneal reinforcement using an acellular dermal matrix for an analysis of biocompatibility, mechanical properties, and transparency
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 8, Issue 9, September 2012, Pages 3326–3332
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering