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A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency

Paper ID Volume ID Publish Year Pages File Format Full-Text
6128 463 2013 12 PDF Available
Title
A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency
Abstract

The low toxicity and efficient gene delivery of polymeric vectors remain the major barrier to the clinical application of non-viral gene therapy. Here, we present a poly-d, l-succinimide (PSI)-based biodegradable cationic polymer which mimicked the golden standard, branched polyethylenimine (PEI, ∼25 kDa). To investigate the influence of 1°, 2°, 3° amine group ratio in the polymer, a series of PSI-based vectors (PSI-NN′x-NNy) grafted with different amine side chains of N,N-dimethyldipropylenetriamine (NN’) and bis(3-aminopropyl)amine (NN) were first characterized and contrasted by biophysical measurements. The in vitro and in vivo biological assay demonstrated that PSI-NN′0.85-NN1 exhibited better transfection ability and biocompatibility than PEI. The present results suggest that such PEI-mimic biodegradable PSI-NN′0.85-NN1 possesses a good potential application for clinical gene delivery.

Keywords
Gene delivery; Vector; Polyethylenimine; Biodegradable; Mimic
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A polyethylenimine-mimetic biodegradable polycation gene vector and the effect of amine composition in transfection efficiency
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 18, June 2013, Pages 4520–4531
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us