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Polymer-based mesh as supports for multi-layered 3D cell culture and assays

Paper ID Volume ID Publish Year Pages File Format Full-Text
6215 468 2014 10 PDF Available
Title
Polymer-based mesh as supports for multi-layered 3D cell culture and assays
Abstract

Three-dimensional (3D) culture systems can mimic certain aspects of the cellular microenvironment found in vivo, but generation, analysis and imaging of current model systems for 3D cellular constructs and tissues remain challenging. This work demonstrates a 3D culture system–Cells-in-Gels-in-Mesh (CiGiM)–that uses stacked sheets of polymer-based mesh to support cells embedded in gels to form tissue-like constructs; the stacked sheets can be disassembled by peeling the sheets apart to analyze cultured cells—layer-by-layer—within the construct. The mesh sheets leave openings large enough for light to pass through with minimal scattering, and thus allowing multiple options for analysis—(i) using straightforward analysis by optical light microscopy, (ii) by high-resolution analysis with fluorescence microscopy, or (iii) with a fluorescence gel scanner. The sheets can be patterned into separate zones with paraffin film-based decals, in order to conduct multiple experiments in parallel; the paraffin-based decal films also block lateral diffusion of oxygen effectively. CiGiM simplifies the generation and analysis of 3D culture without compromising throughput, and quality of the data collected: it is especially useful in experiments that require control of oxygen levels, and isolation of adjacent wells in a multi-zone format.

Keywords
ECM (extracellular matrix); 3D tumor model; Oxygen gradient; Surface modification; Composite material; Hydrogels
First Page Preview
Polymer-based mesh as supports for multi-layered 3D cell culture and assays
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 1, January 2014, Pages 259–268
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering