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Polycation-functionalized nanoporous silicon particles for gene silencing on breast cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
6232 468 2014 9 PDF Available
Title
Polycation-functionalized nanoporous silicon particles for gene silencing on breast cancer cells
Abstract

Nanoporous silicon particles (pSi), with a pore size in the range of 20–60 nm, were modified with polyethyleneimine (PEI) to yield pSi–PEI particles, which were subsequently complexed with siRNA. Thus, pSi–PEI/siRNA particles were fabricated, with the PEI/siRNA nanocomplexes mainly anchored inside the nanopore of the pSi particles. These hybrid particles were used as carriers to deliver siRNA to human breast cancer cells. Due to the gradual degradation of the pSi matrix under physiological conditions, the PEI/siRNA nanocomplexes were released from the pore interior in a sustained manner. Physicochemical characterization revealed that the released PEI/siRNA nanocomplexes exhibited well-defined spherical shape and narrow particle size distribution between 15 and 30 nm. Gene knockdown against the ataxia telangiectasia mutated (ATM) cancer gene showed dramatic gene silencing efficacy. Moreover, comprehensive biocompatibility studies were performed for the pSi–PEI/siRNA particles both in vitro and in vivo and demonstrated that the pSi–PEI particles exhibited significantly enhanced biocompatibility. As a consequence, PEI-modified porous silicon particles may have substantial potential as safe and effective siRNA delivery systems.

Keywords
Non-viral gene delivery; Polyplex; siRNA; Porous silicon particles; Breast cancer
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Polycation-functionalized nanoporous silicon particles for gene silencing on breast cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 35, Issue 1, January 2014, Pages 423–431
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us