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Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T

Paper ID Volume ID Publish Year Pages File Format Full-Text
6266 471 2013 10 PDF Available
Title
Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T
Abstract

Fe3O4@SiO2 core–shell nanoparticles were synthesized and used to label human mesenchymal stem cells (hMSCs) for in vitro and in vivo magnetic resonance imaging study. The diameter of the nanoparticles is 24–30 nm with a Fe3O4 core of ∼8 nm and a SiO2 shell of ∼8 nm. Transverse relaxivity of the nanoparticles dispersed in water is measured to be ∼106 mM−1 s−1. After incubation with hMSCs for 12 h at a concentration of 100 μg Fe/mL, cellular uptake of Fe3O4@SiO2 is 20–100 pg Fe/cell, which are located predominantly in the cytoplasm of cells. This level of uptake exhibits no significant influence on hMSCs' viability and differentiation. In vitro imaging of Fe3O4@SiO2-labeled hMSCs evenly distributed in agarose gel yields single cell sensitivity at 11.7 T. In vivo imaging of Fe3O4@SiO2-labeled hMSCs injected into the left brain hemisphere of nude mice yields imaging sensitivity of ∼130 hMSCs.

Keywords
MRI; hMSCs; Fe3O4@SiO2; Single cell sensitivity
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Magnetic resonance imaging of Fe3O4@SiO2-labeled human mesenchymal stem cells in mice at 11.7 T
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 12, April 2013, Pages 3010–3019
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us