fulltext.study @t Gmail

The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
6290 476 2013 9 PDF Available
Title
The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice
Abstract

To improve skin flap healing, one promising strategy in reconstructive surgery might be to optimize platelet rich plasma (PRP) bioactivity and the ischemia-altered expression of genes. We studied both the effect of PRP on ischemic flaps, and whether in vivo bioluminescence imaging (BLI) is a suitable method for the longitudinal monitoring of angiogenesis in surgical wounds. Axial murine skin flaps were created in four experimental groups. In vivo measurements of VEGFR2 expression levels were made every other day until the 14th day. The local VEGF level and microvessel density were quantified on the 14th day via ELISA and immunohistochemistry, and flap survival rates were measured. We demonstrated that PRP and induced ischemia have a beneficial influence on angiogenesis and flap healing. Combining the two resulted in a significantly robust increase in angiogenesis and flap survival rate that was corroborated by bioluminescence imaging of VEGFR2 activity. This study shows that angiogenic effects of PRP may be potentialized by the stimulus of induced ischemia during free flap harvesting, and thus the two procedures appear to have a synergistic effect on flap healing. This study further demonstrates that BLI of modulated genes in reconstructive surgery is a valuable model for longitudinal in vivo evaluation of angiogenesis.

Keywords
Platelet rich plasma; Ischemia; VEGF; VEGFR2; Bioluminescence imaging; Free flap
First Page Preview
The effect of platelet rich plasma on angiogenesis in ischemic flaps in VEGFR2-luc mice
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 11, April 2013, Pages 2674–2682
Authors
, , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering