fulltext.study @t Gmail

The generation of pancreatic β-cell spheroids in a simulated microgravity culture system

Paper ID Volume ID Publish Year Pages File Format Full-Text
6314 480 2013 7 PDF Available
Title
The generation of pancreatic β-cell spheroids in a simulated microgravity culture system
Abstract

Islet transplantation can induce a substantial improvement in the treatment of type 1 diabetes mellitus. However, the clinical application of islet transplantation is severely limited by the shortage of donor organs. It is thus essential to improve the engraftment rate to achieve the expected outcome in the treatment of diabetes mellitus using a limited amount of donor islets. In this manuscript, we describe the generation of β-cell spheroids using mouse insulinoma cells (MIN6) as a model of β-cells. We established a 3D culture system that simulates microgravity using a 3D clinostat. Using this method, we were able to produce 100 spheroids per mL of culture media. The optimization of the culture conditions in the clinostat produced spheroids with a size of approximately 250 μm, which is a size that is known to induce good graft survival after islet transplantation. The spheroids produced in the clinostat expressed several β-cell signature genes at higher levels than the levels that were found in MIN6 cells that were cultured in a standard 2D culture dish (MIN6-2D). The transplantation of the spheroids into the portal vein of streptozotocin-induced diabetic mice ameliorates hyperglycemia, whereas the transplantation of the equivalent number of 2D-cultured cells failed to cure diabetes. These results indicate that the clinostat culture provides a new method for the reconstitution of a large number of functional β-cell spheroids for diabetes treatment.

Keywords
Islet; Clinostat; Diabetes; Islet transplantation
First Page Preview
The generation of pancreatic β-cell spheroids in a simulated microgravity culture system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 23, July 2013, Pages 5785–5791
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us