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The application of pH-sensitive polymer-lipids to antigen delivery for cancer immunotherapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
6370 485 2013 11 PDF Available
Title
The application of pH-sensitive polymer-lipids to antigen delivery for cancer immunotherapy
Abstract

For production of pH-sensitive liposomes, we developed pH-sensitive polymer-lipids that consists of pH-sensitive fusogenic polymer moieties such as 3-methyl glutarylated poly(glycidol) and 2-carboxycyclohexane-1-carboxylated poly(glycidol), connected to a phosphatidylethanolamine head group. Incorporation of these pH-sensitive polymer-lipids into egg yolk phosphatidylcholine liposomes produced highly pH-sensitive liposomes that were stable at neutral pH but which destabilized markedly in response to very small pH change in weakly acidic pH region. These liposomes delivered their contents (pyranine) into cytosol of dendritic cell-derived DC2.4 cells. When these polymer-lipid-incorporated liposomes loaded with antigenic protein ovalbumin (OVA) were administered subcutaneously to mice, the antigen-specific cellular immunity was induced efficiently in the mice. Furthermore, immunization of mice with these OVA-loaded pH-sensitive polymer-lipid-incorporated liposomes induced strong OVA-specific immunity, which achieved complete rejection of OVA-expressing E.G7-OVA cells and marked regression of E.G7-OVA tumors.

Keywords
pH-sensitive polymer; pH-sensitive liposome; Cytoplasmic delivery; Cancer immunotherapy; Dendritic cell; Cellular immunity
First Page Preview
The application of pH-sensitive polymer-lipids to antigen delivery for cancer immunotherapy
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 22, July 2013, Pages 5711–5721
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering