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A multi-structural single cell model of force-induced interactions of cytoskeletal components

Paper ID Volume ID Publish Year Pages File Format Full-Text
6395 489 2013 8 PDF Available
Title
A multi-structural single cell model of force-induced interactions of cytoskeletal components
Abstract

Several computational models based on experimental techniques and theories have been proposed to describe cytoskeleton (CSK) mechanics. Tensegrity is a prominent model for force generation, but it cannot predict mechanics of individual CSK components, nor explain the discrepancies from the different single cell stimulating techniques studies combined with cytoskeleton-disruptors. A new numerical concept that defines a multi-structural 3D finite element (FE) model of a single-adherent cell is proposed to investigate the biophysical and biochemical differences of the mechanical role of each cytoskeleton component under loading. The model includes prestressed actin bundles and microtubule within cytoplasm and nucleus surrounded by the actin cortex. We performed numerical simulations of atomic force microscopy (AFM) experiments by subjecting the cell model to compressive loads. The numerical role of the CSK components was corroborated with AFM force measurements on U2OS-osteosarcoma cells and NIH-3T3 fibroblasts exposed to different cytoskeleton-disrupting drugs. Computational simulation showed that actin cortex and microtubules are the major components targeted in resisting compression. This is a new numerical tool that explains the specific role of the cortex and overcomes the difficulty of isolating this component from other networks in vitro. This illustrates that a combination of cytoskeletal structures with their own properties is necessary for a complete description of cellular mechanics.

Keywords
Cytoskeleton; Finite element modeling; Actin cortex; Actin bundles; Microtubules; AFM (atomic force microscopy)
First Page Preview
A multi-structural single cell model of force-induced interactions of cytoskeletal components
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 26, August 2013, Pages 6119–6126
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering