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Single-molecule level binding force between collagen and collagen binding domain-growth factor conjugates

Paper ID Volume ID Publish Year Pages File Format Full-Text
6398 489 2013 8 PDF Available
Title
Single-molecule level binding force between collagen and collagen binding domain-growth factor conjugates
Abstract

Biological studies have shown that collagen/collagen binding domain (CBD)-growth factor composites are effective biomaterials systems for tissue engineering and regeneration. Here we present atomic force spectroscopy (AFM)-based investigations at the single molecule level to address fundamental biophysical questions such as CBD binding sites distribution and the mechanism for controlled release of growth factors from the collagen scaffold. Non-uniformly distributed CBD binding sites on collagen membrane are directly visualized with a quantum dot-based bimodal imaging method. AFM force spectroscopy unbinding experiments reveal that modest unbinding force and dissociation constant of the CBD/collagen interaction could be the key for its successful application in controlled release systems.

Keywords
Atomic force microscopy; Collagen membrane; Growth factor; Collagen binding domain; Dissociation constant; AFM force spectroscopy
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Single-molecule level binding force between collagen and collagen binding domain-growth factor conjugates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 26, August 2013, Pages 6139–6146
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us