fulltext.study @t Gmail

The critical role of ECM proteins within the human MSC niche in endothelial differentiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
6415 490 2013 12 PDF Available
Title
The critical role of ECM proteins within the human MSC niche in endothelial differentiation
Abstract

Interactions between blood vessels and osteoblasts—bone-forming cells—are critical for successful bone development. We therefore investigated the endothelial differentiation capacity of mesenchymal stem cells (MSCs) derived from bone tissue. We found that fetal pre-osteoblast and adult trabecular bone-derived (TB) MSCs express similar surface markers as bone marrow (BM) MSCs and can differentiate into adipocytes, osteoblasts, and chondrocytes. However, when cultured in extracellular matrix (ECM) and endothelial differentiation conditions, bone-derived MSCs (B-MSCs) more readily form tubular structures and uptake acetylated low-density lipoproteins, fulfilling the functional criteria for endothelial cells (ECs). Moreover, addition of B-MSCs but not other cells significantly enhanced vessel formation in the in vivo chick chorioallantoic membrane assay. Mechanistically, this appears to be due to the upregulation of the endothelial transcription factor forkhead box protein C2 (FOXC2) and its downstream gene αvβ3 integrin/CD61in B-MSCs but not BMMSCs by laminin, a component protein of the ECM. Our findings not only reveal discrepant differentiation capacity for various tissue-specific MSCs, but also highlight the critical role of the niche—in this case, the ECM and its component proteins—in determining lineage commitment of stem cells.

Keywords
Mesenchymal stem cells; Bone; Endothelial differentiation; Extracellular matrix; FOXC2; Integrin αvβ3/CD61
First Page Preview
The critical role of ECM proteins within the human MSC niche in endothelial differentiation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 17, June 2013, Pages 4223–4234
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering