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Peptide-mediated intracellular delivery of miRNA-29b for osteogenic stem cell differentiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
6428 490 2013 13 PDF Available
Title
Peptide-mediated intracellular delivery of miRNA-29b for osteogenic stem cell differentiation
Abstract

Stem cell differentiation is modulated by several key molecules, including cytokines, hormones, and engineered peptides. Emerging evidence suggests that microRNA has potential applications in stem cell engineering, such as in osteoblastic differentiation. MicroRNAs (miRNAs) bind to the 3′-untranslated region (UTR) sequence of target mRNA, thereby attenuating protein synthesis. Our goal was to evaluate the delivery of miRNA, i.e., miRNA-29b, to stem cells to promote osteoblastic differentiation because this miRNA is known to target anti-osteogenic factors gene expression. Despite the important role of miRNAs, their application has been limited due to poor cell/tissue penetration. The authors attempted to overcome this limitation by using a cell-penetrating peptide (CPP) carrier. Herein, the arginine-rich CPP, called the lowmolecular weight protamine (LMWP), is the sequence from natural protamine. We worked out the difficult problem to transfect into hMSCs by the complex with LMWP, and then we investigated synthetic double-stranded miR-29b could be induced osteoblast differentiation.

Keywords
MicroRNA-29b; Cell-penetrating peptide; Human mesenchymal stem cells; Non-toxic intracellular transfection; Osteogenic differentiation
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Peptide-mediated intracellular delivery of miRNA-29b for osteogenic stem cell differentiation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 17, June 2013, Pages 4347–4359
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us