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Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
6440 491 2013 12 PDF Available
Title
Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix
Abstract

Mesenchymal stem cells (MSCs) have gained increasing research interest for their potential in improving healing and regeneration of injured tendon tissues. Developing functional three-dimensional (3D) scaffolds to promote MSC proliferation and differentiation is a critical requirement in tendon tissue engineering. Tendon extracellular matrix has been shown to maintain the tenogenic potential of tendon stem cells and stimulate tenogenesis of human adipose stem cells (hASCs) in 2D culture. This study aims at characterizing the biological composition of urea-extracted fraction of tendon ECM (tECM) and its tenogenic effect on hASCs cultured in a 3D collagen scaffold under uniaxial tension. The tECM obtained was cell-free and rich in ECM proteins. hASCs seeded in tECM-supplemented scaffold exhibited significantly increased proliferation and tenogenic differentiation. The presence of tECM also greatly suppressed the osteogenic differentiation of hASCs triggered by uniaxial tension. In addition, tECM-supplemented constructs displayed enhanced mechanical strength, accompanied by reduced expression and activity of MMPs in the seeded hASCs, indicating a regulatory activity of tECM in cell-mediated scaffold remodeling. These findings support the utility of tECM in creating bio-functional scaffolds for tendon tissue engineering.

Keywords
Decellularized matrix; Adipose stem cells; Collagen scaffold; Tenogenesis; Tendon regeneration
First Page Preview
Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 37, December 2013, Pages 9295–9306
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering