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The osteogenic response of mesenchymal stem cells to an injectable PLGA bone regeneration system

Paper ID Volume ID Publish Year Pages File Format Full-Text
6445 491 2013 13 PDF Available
Title
The osteogenic response of mesenchymal stem cells to an injectable PLGA bone regeneration system
Abstract

The enrichment of substrates/surfaces with selected functional groups, methyl (–CH3), allyl amine (–NH2), allyl alcohol (–OH) and acrylic acid (–COOH), can be used to trigger mesenchymal stem (MSC) cell differentiation into specified lineages, minimising the need for exogenous biological supplementation. We present the successful translation of this research phenomenon to an injectable two phase injectable PLGA system, utilising plasma techniques, for the repair of bone defects. Modified microspheres were characterised using water contact angel (WCA), X-ray Photon Spectroscopy (XPS) and scanning electron microscopy (SEM). When cultured in contact with MSCs in vitro, the ability of the modified particles, within the 2 phase system, to induce differentiation was characterised using quantitative assays for cell viability and histological analysis for key markers of differentiation throughout the entirety of the three dimensional scaffold. Biological analysis proved that selected modified microspheres have the ability to induce MSC osteogenic (-NH2 modified scaffolds) and chondrogenic (–OH modified scaffolds) differentiation throughout the entirety of the formed scaffold. Therefore optimised plasma modification of microspheres is an effective tool for the production of injectable systems for the repair of bone and cartilage defects.

Keywords
Mesenchymal stem cells; PLGA; Osteogenic; Degradable polymer
First Page Preview
The osteogenic response of mesenchymal stem cells to an injectable PLGA bone regeneration system
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 37, December 2013, Pages 9352–9364
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering