fulltext.study @t Gmail

Discarded human kidneys as a source of ECM scaffold for kidney regeneration technologies

Paper ID Volume ID Publish Year Pages File Format Full-Text
6515 494 2013 11 PDF Available
Title
Discarded human kidneys as a source of ECM scaffold for kidney regeneration technologies
Abstract

In the United States, more than 2600 kidneys are discarded annually, from the total number of kidneys procured for transplant. We hypothesized that this organ pool may be used as a platform for renal bioengineering and regeneration research. We previously showed that decellularization of porcine kidneys yields renal extracellular matrix (ECM) scaffolds that maintain their basic components, support cell growth and welfare in vitro and in vivo, and show an intact vasculature that, when such scaffolds are implanted in vivo, is able to sustain physiological blood pressure. The purpose of the current study was to test if the same strategy can be applied to discarded human kidneys in order to obtain human renal ECM scaffolds. The results show that the sodium dodecylsulfate-based decellularization protocol completely cleared the cellular compartment in these kidneys, while the innate ECM framework retained its architecture and biochemical properties. Samples of human renal ECM scaffolds stimulated angiogenesis in a chick chorioallantoic membrane assay. Importantly, the innate vascular network in the human renal ECM scaffolds retained its compliance. Collectively, these results indicate that discarded human kidneys are a suitable source of renal scaffolds and their use for tissue engineering applications may be more clinically applicable than kidneys derived from animals.

Keywords
Discarded kidneys; Renal transplantation; Organ bioengineering and regeneration; Scaffold; Decellularization; BiomaterialECM, extracellular matrix; OBR, organ bioengineering and regeneration; SEM, scanning electronic microscopy; SDS, sodium dodecylsulfate
First Page Preview
Discarded human kidneys as a source of ECM scaffold for kidney regeneration technologies
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 24, August 2013, Pages 5915–5925
Authors
, , , , , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering