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Targeting delivery of saquinavir to the brain using 83-14 monoclonal antibody-grafted solid lipid nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
6536 499 2013 13 PDF Available
Title
Targeting delivery of saquinavir to the brain using 83-14 monoclonal antibody-grafted solid lipid nanoparticles
Abstract

83-14 monoclonal antibody (MAb) was modified on solid lipid nanoparticles (SLNs) to improve the brain-targeting delivery of saquinavir (SQV). The endocytosis of 83-14 MAb-grafted SQV-loaded SLNs (83-14 MAb/SQV-SLNs) into human brain-microvasscular endothelial cells (HBMECs) was studied by staining cell nuclei, insulin receptors, and drug carriers. An increase in the weight fraction of palmitic acid in lipid core enhanced the particle size, absolute value of zeta potential, and viability of HBMECs and reduced the entrapment efficiency and release rate of SQV. In addition, an increase in the weight fraction of poloxamer 407 in surfactant layer reduced the particle size, absolute value of zeta potential, phagocytosis by RAW246.7 cells, permeability across the blood–brain barrier (BBB), and uptake by HBMECs and enhanced the viability of HBMECs. Moreover, an increase in the concentration of surface 83-14 MAb enhanced the percentage of surface nitrogen, permeability across the BBB, and uptake by HBMECs and did not significantly vary the viability of HBMECs and phagocytosis by RAW264.7 cells. 83-14 MAb/SQV-SLNs can ameliorate the bioavailability characteristics of SQV, inhibit the lymphatic particle uptake, and promote the transport of SQV into brain endothelia.

Keywords
83-14 Monoclonal antibody; Solid lipid nanoparticle; Saquinavir; Brain; Endothelium; Phagocytosis
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Targeting delivery of saquinavir to the brain using 83-14 monoclonal antibody-grafted solid lipid nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 20, July 2013, Pages 4818–4830
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us