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The switching of focal adhesion maturation sites and actin filament activation for MSCs by topography of well-defined micropatterned surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
6545 500 2013 8 PDF Available
Title
The switching of focal adhesion maturation sites and actin filament activation for MSCs by topography of well-defined micropatterned surfaces
Abstract

Securing robust cell adhesion between cells and biomaterials is one of key considerations for tissue engineering. However, the cell adhesion investigation by the biophysical effects such as topography or rigidity of substrates has only been recently reported. In this study, we examined the spatial property of focal adhesions by changing the height of micropatterns in two kinds of microtopography (grid and post) and the stiffness of the substrates. We found that the focal adhesion localization is highly regulated by topographical variation (height) of gird micropattens but not the rigidity of substrates or the function of actin cytoskeleton, although the latters strongly influence the focal adhesion size or area. In detail, the change of the height of the grid micropatterns results in the switching of focal adhesion sites; as the height increases, the localization of focal adhesion is switched from top to bottom areas. This study demonstrates that the localization of focal adhesion on well-defined micropatterned substrates is critically determined by the topographical variation in the micropatterns.

Keywords
Topography; Focal adhesion; Focal adhesion kinase; Vinculin; Actin filament
First Page Preview
The switching of focal adhesion maturation sites and actin filament activation for MSCs by topography of well-defined micropatterned surfaces
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 7, February 2013, Pages 1764–1771
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering