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Endocytosis, intracellular transport, and exocytosis of lanthanide-doped upconverting nanoparticles in single living cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
6603 502 2012 7 PDF Available
Title
Endocytosis, intracellular transport, and exocytosis of lanthanide-doped upconverting nanoparticles in single living cells
Abstract

Lanthanide-doped upconverting nanoparticles (UCNPs) have recently attracted enormous attention in the field of biological imaging owing to their unique optical properties (near-infrared excitation followed by photoluminescence in the visible spectral range). For biological applications, it is critical to understand the interaction between these nanoparticles and biological systems at the cellular level. In this paper, using epi-fluorescence microscopy with 980-nm excitation, a full intracellular pathway composed of endocytosis, active transport, and exocytosis was clearly visualized for PEG-phospholipid-coated UCNPs in single HeLa cells, which was experimentally feasible mostly thanks to the excellent photostability and low cytotoxicity thereof. Each step in the pathway was characterized and identified by various chemical inhibition studies and spectroscopic measurements.

Keywords
Nanoparticle; Image analysis; Fluorescence; Molecular imaging; Cytotoxicity
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Endocytosis, intracellular transport, and exocytosis of lanthanide-doped upconverting nanoparticles in single living cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 35, December 2012, Pages 9080–9086
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us