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Gas-filled microbubble-mediated delivery of antigen and the induction of immune responses

Paper ID Volume ID Publish Year Pages File Format Full-Text
6619 506 2012 12 PDF Available
Title
Gas-filled microbubble-mediated delivery of antigen and the induction of immune responses
Abstract

The use of well characterized recombinant or purified protein antigens (Ag) for vaccination is of interest for safety reasons and in the case where inactivated pathogens are not available (cancer, allergy). However it requires the addition of adjuvants such as Ag carrier or immune stimulators to potentiate their immunogenicity. In this study, we demonstrated that gas-filled microbubbles (MB) can serve as an efficient Ag delivery system to promote phagocytosis of the model Ag ovalbumin (OVA) without the need of ultrasound application. Once internalized by DC, OVA was processed and presented to both CD4 and CD8 T cells in vitro; such observations were coupled with the capacity of MB to activate DC. In vivo administration of MB-associated OVA in naïve wild-type Balb/c mice resulted in the induction of OVA-specific antibody and T cell responses. Detailed characterization of the generated immune response demonstrated the production of both IgG1 and IgG2a serum antibodies, as well as the secretion of IFN-γ and IL-10 by splenocytes. Interestingly, similar results were obtained with human DC in regards of Ag delivery and cell activation. Therefore, the data presented here settle the proof of principle for the further evaluation of MB-based immunomodulation studies.

Keywords
Microparticles; Gas-filled microbubbles; Dendritic cells; Antigen delivery; Antibodies; T cell immunityMB, gas-filled microbubbles; P-MB, plain MB; OVA-MB, OVA covalently linked to MB; Cy3:OVA, Cy3-labeled OVA; MFI, mean fluorescence intensity; APC, antig
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Gas-filled microbubble-mediated delivery of antigen and the induction of immune responses
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 25, September 2012, Pages 5935–5946
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us