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Using genipin-crosslinked acellular porcine corneal stroma for cosmetic corneal lens implants

Paper ID Volume ID Publish Year Pages File Format Full-Text
6640 510 2012 11 PDF Available
Title
Using genipin-crosslinked acellular porcine corneal stroma for cosmetic corneal lens implants
Abstract

Acellular porcine corneal stroma (APCS) has been proven to maintain the matrix microenvironment and is therefore an ideal biomaterial for the repair and reconstruction of corneal stroma. This study aims to develop a method to prepare cosmetic corneal lens implants for leukoma using genipin-crosslinked APCS (Gc-APCS). The Gc-APCS was prepared from APCS immersed in 1.0% genipin aqueous solution (pH 5.5) for 4 h at 37 °C, followed by lyophilization at −10 °C. The color of the Gc-APCS gradually deepened to dark-blue. The degree of crosslinking was 45.7 ± 4.6%, measured by the decrease of basic and hydroxy amino acids. The porous structure and ultrastructure of collagenous lamellae were maintained, and the porosity and BET SSA were 72.7 ± 4.6% and 23.01 ± 3.45 m2/g, respectively. The Gc-APCS rehydrated to the physiological water content within 5 min and was highly resistant to collagenase digestion. There were no significant differences in the areal modulus and curvature variation between Gc-APCS and nature porcine cornea. The dark-blue pigments were stable to pH, light and implantation in vivo. Gc-APCS extracts had no inhibitory effects on the proliferation of keratocytes. Corneal neovascularization, graft degradation and corneal rejection were not observed within 6 months.

Keywords
Corneal tissue engineering; Scaffold; Decellularization; Crosslinking; Genipin
First Page Preview
Using genipin-crosslinked acellular porcine corneal stroma for cosmetic corneal lens implants
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 30, October 2012, Pages 7336–7346
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering