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Controlling Pseudomonas aeruginosa persister cells by weak electrochemical currents and synergistic effects with tobramycin

Paper ID Volume ID Publish Year Pages File Format Full-Text
6642 510 2012 10 PDF Available
Title
Controlling Pseudomonas aeruginosa persister cells by weak electrochemical currents and synergistic effects with tobramycin
Abstract

It is well recognized that bacterial populations commonly contain a small percentage of phenotypic variants, known as persister cells, which are dormant and extremely tolerant to antibiotics. When the antibiotic treatment is stopped, surviving persister cells can regenerate the bacterial population with a similar percentage of persister cells. Such persistence presents a great challenge to curing chronic infections, such as those associated with implanted medical devices. In this study, we report that bacterial persister cells can be effectively eliminated by low-level direct currents (DCs); e.g. treatment with 70 μA/cm2 DC for 1 h using stainless steel (SS) 304 reduced the number of viable planktonic persister cells of Pseudomonas aeruginosa PAO1 by 98% compared to the untreated control. In addition to persister killing by applying DC alone, synergistic effects were observed when treating persister cells with 70 μA/cm2 DC and 1.5 μg/mL tobramycin together using SS304 electrodes. The same level of DC was also found to be cidal to biofilms-associated persister cells of P. aeruginosa PAO1. These results are helpful for developing more effective methods to control chronic infections associated with implanted medical devices.

Keywords
Bacteria; Persister cells; Biofilm; Electrode; Electrochemical control
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Controlling Pseudomonas aeruginosa persister cells by weak electrochemical currents and synergistic effects with tobramycin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 30, October 2012, Pages 7356–7365
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us