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The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings

Paper ID Volume ID Publish Year Pages File Format Full-Text
6680 511 2012 11 PDF Available
Title
The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings
Abstract

In this study, the effect of ordered rod-like FA coatings of metal discs on adipose-derived stem cell (ASC)’s growth, differentiation and mineralization was studied in vitro; and their mineral inductive effects in vivo. After 3 and 7 days, the cell number on the metal surfaces was significantly higher than those on the ordered and disordered FA surfaces. However, after 4 weeks much greater amounts of mineral formation was induced on the two FA surfaces with and even without osteogenesis induction. The osteogenic profiles showed the up regulation of a set of pro-osteogenic transcripts and bone mineralization phenotypic markers when the ASCs were grown on FA surfaces compared to metal surfaces at 7 and 21 days. In addition to BMP and TGFβ signaling pathways, EGF and FGF pathways also appeared to be involved in ASC differentiation and mineralization. In vivo studies showed accelerated and enhanced mineralized tissue formation integrated within ordered FA coatings. After 5 weeks, over 80% of the ordered FA coating was integrated with a mineralized tissue layer covering the implants. Both the intrinsic properties of the FA crystals and the topography of the FA coating appeared to dominate the cell differentiation and mineralization process.

Keywords
Fluorapatite; Differentiation; Mineralization; Gene profile; Stem cells
First Page Preview
The stimulation of adipose-derived stem cell differentiation and mineralization by ordered rod-like fluorapatite coatings
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 20, July 2012, Pages 5036–5046
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering