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Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling

Paper ID Volume ID Publish Year Pages File Format Full-Text
6691 511 2012 8 PDF Available
Title
Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling
Abstract

Signaling between cells guides biological phenotype. Communications between individual cells, clusters of cells and populations exist in complex networks that, in sum, guide behavior. There are few experimental approaches that enable high content interrogation of individual and multicellular behaviors at length and time scales commensurate with the signal molecules and cells themselves. Here we present “biofabrication” in microfluidics as one approach that enables in-situ organization of living cells in microenvironments with spatiotemporal control and programmability. We construct bacterial biofilm mimics that offer detailed understanding and subsequent control of population-based quorum sensing (QS) behaviors in a manner decoupled from cell number. Our approach reveals signaling patterns among bacterial cells within a single biofilm as well as behaviors that are coordinated between two communicating biofilms. We envision versatile use of this biofabrication strategy for cell–cell interaction studies and small molecule drug discovery.

Keywords
Biofabrication; Biofilm mimics; Cell signaling; Quorum sensing; Chitosan membrane; Alginate hydrogel
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Biofabrication of stratified biofilm mimics for observation and control of bacterial signaling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 20, July 2012, Pages 5136–5143
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us