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Modulation of chondrocyte behavior through tailoring functional synthetic saccharide–peptide hydrogels

Paper ID Volume ID Publish Year Pages File Format Full-Text
6703 513 2012 9 PDF Available
Title
Modulation of chondrocyte behavior through tailoring functional synthetic saccharide–peptide hydrogels
Abstract

Tailoring three-dimensional (3D) biomaterial environments to provide specific cues in order to modulate function of encapsulated cells could potentially eliminate the need for addition of exogenous cues in cartilage tissue engineering. We recently developed saccharide–peptide copolymer hydrogels for cell culture and tissue engineering applications. In this study, we aim to tailor our saccharide–peptide hydrogel for encapsulating and culturing chondrocytes in 3D and examine the effects of changing single amino acid moieties differing in hydrophobicity/hydrophilicity (valine (V), cysteine (C), tyrosine (Y)) on modulation of chondrocyte function. Encapsulated chondrocytes remained viable over 21 days in vitro. Glycosaminoglycan and collagen content was significantly higher in Y-functionalized hydrogels compared to V-functionalized hydrogels. Extensive matrix accumulation and concomitant increase in mechanical properties was evident over time, particularly with the presence of Y amino acid. After 21 days in vitro, Y-functionalized hydrogels attained a modulus of 193 ± 46 kPa, compared to 44 ± 21 kPa for V-functionalized hydrogels. Remarkably, mechanical and biochemical properties of chondrocyte-laden hydrogels were modulated by change in a single amino acid moiety. This unique property, combined with the versatility and biocompatibility, makes our saccharide–peptide hydrogels promising candidates for further investigation of combinatorial effects of multiple functional groups on controlling chondrocyte and other cellular function and behavior.

Keywords
Tissue engineering; Chondrocyte; Cartilage; Sacchride–peptide; Amino acid; Functional group
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Modulation of chondrocyte behavior through tailoring functional synthetic saccharide–peptide hydrogels
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 26, September 2012, Pages 6052–6060
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us