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Criteria impacting the cellular uptake of nanoparticles: A study emphasizing polymer type and surfactant effects

Paper ID Volume ID Publish Year Pages File Format Full-Text
672 57 2011 9 PDF Available
Title
Criteria impacting the cellular uptake of nanoparticles: A study emphasizing polymer type and surfactant effects
Abstract

A detailed understanding of the particle–cell interaction is essential and of immense interest in order to create a “specific carrier” for each particular application. In this paper, the effect of the surfactant type (non-ionic vs ionic) and polymer nature on the cellular uptake of fluorescent polystyrene and poly(l-lactide) nanoparticles was studied on HeLa cells. Nanoparticles in a size range from 100 to 160 nm were synthesized by the miniemulsion process. The particles were detected in cells by confocal laser scanning fluorescence microscopy and flow cytometry. It was found that the influence of the surface charge is greater than that of the polymer type itself. In fact, particles stabilized with cationic surfactant were incorporated in a large number irrespective of polymer type. Cellular pathways at ultrastructural level were studied by transmission electron microscopy in more detail to shed light on the particle–cell interaction based on the material properties. The criteria governing the cellular uptake of nanoparticles based on the polymer and surfactant types are finally established.

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Keywords
Nanoparticle–cell interaction; Surfactant; HeLa cells; Flow cytometry; Electron microscopy
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Criteria impacting the cellular uptake of nanoparticles: A study emphasizing polymer type and surfactant effects
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 12, December 2011, Pages 4160–4168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us