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Light-induced cell detachment for cell sheet technology

Paper ID Volume ID Publish Year Pages File Format Full-Text
6732 514 2013 8 PDF Available
Title
Light-induced cell detachment for cell sheet technology
Abstract

The phenomenon of light-induced cell detachment is reported. Mouse calvaria-derived, pre-osteoblastic (MC3T3-E1) cells were cultured on a TiO2 nanodot-coated quartz substrate. After 20 min of UV365 illumination, over 90% of the cells would detach from the surface. Moreover, intact cell sheets could be obtained in the same way. It was found that the as-obtained cells showed good viability, and could be used for further culture processes and other applications. Also, biocompatibility and safety characterizations indicated that the use of TiO2 nanodots and UV365 illumination was safe for such cell detachment. It is suggested that adsorbed extracellular matrix proteins play key roles in developing cell sheets and ensuring biocompatibility. The present light-induced cell detachment method demonstrates a promising way for rapid cell/cell sheet harvesting.

Keywords
Cell detachment; Light-induced; Titanium dioxide; Nanodots film
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Light-induced cell detachment for cell sheet technology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 1, January 2013, Pages 11–18
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us