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The regulation of epithelial cell proliferation and growth by IL-1 receptor antagonist

Paper ID Volume ID Publish Year Pages File Format Full-Text
6742 514 2013 9 PDF Available
Title
The regulation of epithelial cell proliferation and growth by IL-1 receptor antagonist
Abstract

We have performed clinical translation of epithelial cell sheets fabricated on temperature-responsive culture surfaces to treat cornea and esophagus. In the preclinical study using animal models, we found epithelial cell growth potential varied among species. Canine epithelial cell growth was prominent, while rat one was poor under 3T3 feeder layer-free condition. The aim of the present study was to identify growth-promoting factors for epithelial cells. Conditioned medium of canine cell culture harvested at different time points showed different growth promotive activity for rat epithelial cells. Time-dependent gene expression was quantitatively evaluated for forty growth factors, and compared with conditioned medium results. Statistically significant promotive activity was observed with IL-1RA, and significant inhibitory activity was observed with IL-1α. Furthermore, neutralizing anti-IL-1α antibody also showed significant promotive activity. Human epidermal keratinocytes were promoted to proliferate by IL-1RA and neutralizing anti-IL-1α antibody, and showed well differentiation to form transplantable, squamous stratified epithelial cell sheets. These findings would be useful to fabricate reproducible, transplantable epithelial cell sheets for regenerative medicine.

Keywords
Cell proliferation; Epithelial cell; Stem cell; Growth factors; Interleukin; Gene expression
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 1, January 2013, Pages 121–129
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us