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The effect of adenovirus-mediated siRNA targeting BMPR-II on UHMWPE-induced osteoclast formation

Paper ID Volume ID Publish Year Pages File Format Full-Text
6745 514 2013 10 PDF Available
Title
The effect of adenovirus-mediated siRNA targeting BMPR-II on UHMWPE-induced osteoclast formation
Abstract

Aseptic loosening (AL) is the single most common complication of total joint arthroplasty. The critical factor may contribute to loosening is the adverse tissue response to wear debris. A growing body of literature suggests that BMPs influence the formation and activity of osteoclasts, and BMP signaling plays an important role in the osteoclast formation. In this study, we have employed an RNA interference approach by transfecting a small interfering RNA (siRNA) specific for BMPR-II, to determine the possible importance of this receptor as a target for UHMWPE (Ultra high molecular weight polyethylene) induced osteoclastogenesis in the air pouch model in vivo. Meanwhile, in order to further elucidation of the mechanism of BMPR-II signaling pathway in osteoclast formation, we investigated the effects of siBMPR-II toward RANKL induced osteoclast differentiation in vitro. The present study showed that locally injection of adenovirus-mediated siRNA targeting BMPR-II appears to be a feasible and effective candidate to treat or prevent wear debris-associated osteolysis. Furthermore, we revealed that the effects of BMPR-II signaling on osteoclast formation are mediated directly by osteoclast itself, as well as indirectly by altered expression of RANKL and OPG in osteoblast.

Keywords
BMPR-II; siRNA; UHMWPE; Air pouch model; Osteoclast
First Page Preview
The effect of adenovirus-mediated siRNA targeting BMPR-II on UHMWPE-induced osteoclast formation
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 1, January 2013, Pages 150–159
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering