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Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
675 57 2011 8 PDF Available
Title
Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering
Abstract

A surface modification technique based on poly(dopamine) deposition developed from oxidative polymerization of dopamine is known to promote cell adhesion to several cell-resistant substrates. In this study this technique was applied to articular cartilage tissue engineering. The adhesion and proliferation of rabbit chondrocytes were evaluated on poly(dopamine)-coated polymer films, such as polycaprolactone, poly(l-lactide), poly(lactic-co-glycolic acid) and polyurethane, biodegradable polymers that are commonly used in tissue engineering. Cell adhesion was significantly increased by merely 15 s of dopamine incubation, and 4 min incubation was enough to reach maximal cell adhesion, a 1.35–2.69-fold increase compared with that on the untreated substrates. Cells also grew much faster on the poly(dopamine)-coated substrates than on untreated substrates. The increase in cell affinity for poly(dopamine)-coated substrates was demonstrated via enhancement of the immobilization of serum adhesive proteins such as fibronectin. When the poly(dopamine)-coating technique was applied to three-dimensional (3-D) polyurethane scaffolds, the proliferation of chondrocytes and the secretion of glycosaminoglycans were increased compared with untreated scaffolds. Our results show that the deposition of a poly(dopamine) layer on 3-D porous scaffolds is a simple and promising strategy for articular cartilage tissue engineering, and may be applied to other types of tissue engineering.

Keywords
Chondrocyte; Biodegradable polymer; Dopamine; Glycosaminoglycan; Cell adhesion and proliferation
First Page Preview
Poly(dopamine) coating of scaffolds for articular cartilage tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 12, December 2011, Pages 4187–4194
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering