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Synergistic anticancer effect of RNAi and photothermal therapy mediated by functionalized single-walled carbon nanotubes

Paper ID Volume ID Publish Year Pages File Format Full-Text
6755 514 2013 13 PDF Available
Title
Synergistic anticancer effect of RNAi and photothermal therapy mediated by functionalized single-walled carbon nanotubes
Abstract

Single-walled carbon nanotubes (SWNTs) are special nano-materials which exhibit interesting physical and chemical properties, presenting new opportunities for biomedical research and applications. In this study, we have successfully adopted a novel strategy to chemically functionalize SWNTs with polyethylenimine (PEI) through purification, oxidation, amination and polymerization, which were then bound by DSPE-PEG2000-Maleimide for further conjugation with the tumor targeting NGR (Cys-Asn-Gly-Arg-Cys-) peptide via the maleimide group and sulfhydryl group of cysteine, and finally hTERT siRNA was loaded to obtain a novel tumor targeting siRNA delivery system, designated as SWNT-PEI/siRNA/NGR. The results showed that SWNT-PEI/siRNA/NGR could efficiently cross cell membrane, induced more severe apoptosis and stronger suppression in proliferation of PC-3 cells in vitro. Furthermore, in tumor-bearing mice model the delivery system exhibited higher antitumor activity due to more accumulation in tumor without obvious toxicity in main organs. The combination of RNAi and near-infrared (NIR) photothermal therapy significantly enhanced the therapeutic efficacy. In conclusion, SWNT-PEI/siRNA/NGR is a novel and promising anticancer system by combining gene therapy and photothermal therapy.

Keywords
Single-walled carbon nanotubes; RNAi; Photothermal therapy; Tumor-targeting; NGR
First Page Preview
Synergistic anticancer effect of RNAi and photothermal therapy mediated by functionalized single-walled carbon nanotubes
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 1, January 2013, Pages 262–274
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering