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Rapid aggregation of heterogeneous cells and multiple-sized microspheres in methylcellulose medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
6786 517 2012 7 PDF Available
Title
Rapid aggregation of heterogeneous cells and multiple-sized microspheres in methylcellulose medium
Abstract

We report a method for the rapid production of cellular aggregates without electric power and cell modification. We focused on the swelling property of a solution containing a high molecular material, methylcellulose (MC), which immediately absorbs a small amount of solvent and fills the space occupied by the solvent. When 1 μl of a suspension of 1000 animal cells in normal culture medium was injected into the 3% MC medium, the normal medium was rapidly absorbed by the surrounding MC medium. Suspended cells were simultaneously trapped on the interfaces between the normal and MC media; they were finally pulled together and held in the MC medium. This event was nearly complete within the first 10 min. Moreover, MC medium-dependent aggregation was observed when polystyrene microspheres of different sizes (diameter, 100 nm–100 μm) were added. Furthermore, we demonstrated the stepwise fabrication of multi-layered aggregates with embedded structures. These methods for creating engineered aggregates should enhance the study of three-dimensional cultures comprising two or more cell types with well-designed structures.

Keywords
Swelling; Absorption; Microsphere; Nanoparticle
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Rapid aggregation of heterogeneous cells and multiple-sized microspheres in methylcellulose medium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 18, June 2012, Pages 4508–4514
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us