fulltext.study @t Gmail

Real time observation and kinetic modeling of the cellular uptake and removal of silicon quantum dots

Paper ID Volume ID Publish Year Pages File Format Full-Text
6799 517 2012 7 PDF Available
Title
Real time observation and kinetic modeling of the cellular uptake and removal of silicon quantum dots
Abstract

The time courses of uptake and removal of silicon quantum dots (Si-QDs) by human umbilical endothelial cells (HUVECs) were observed via confocal laser scanning microscope. Si-QDs were internalized via endocytosis and transported to late endosomes/lysosomes. The number of internalized Si-QDs increased with time and gradually reached a plateau value. When Si-QD-internalized HUVECs were subsequently washed and exposed to fresh culture medium, HUVECs removed internalized Si-QDs via exocytosis. The number of internalized Si-QDs decreased with time and gradually reached a plateau value. Not all internalized Si-QDs were removed from the cell interior but large numbers of internalized Si-QDs remained accumulated inside cells. A kinetic model based on the mass balance of Si-QDs and receptors in a cell was proposed to describe the cellular uptake and removal of Si-QDs. Model calculation fitted well with experimental results. Using this model, the dissociation constant between receptors and Si-QDs in the endosome, Kd,in, was found to be a determinant factor for Si-QD accumulation in cells after the removal process.

Keywords
Silicon; Nanoparticle; Fluorescence; Confocal microscopy; Modeling; Endothelial cell
First Page Preview
Real time observation and kinetic modeling of the cellular uptake and removal of silicon quantum dots
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 18, June 2012, Pages 4639–4645
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us