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Promoting engraftment of transplanted neural stem cells/progenitors using biofunctionalised electrospun scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
6817 519 2012 10 PDF Available
Title
Promoting engraftment of transplanted neural stem cells/progenitors using biofunctionalised electrospun scaffolds
Abstract

With the brain's limited capacity for repair, new and innovative approaches are required to promote regeneration. While neural transplantation for a number of neural disease/injuries have been demonstrated, major limitations in the field include poor cell survival and integration. This, in part, is due to the non-conducive environment of the adult brain, failing to provide adequate chemical and physical support for new neurons. Here we examine the capacity of fibrous poly ε-caprolactone (PCL) scaffolds, biofunctionalised with immobilised glial cell-derived neurotrophic factor (GDNF), to influence primary cortical neural stem cells/progenitors in vitro and enhance integration of these cells following transplantation into the brain parenchyma. Immobilisation of GDNF was confirmed prior to in vitro culturing and at 28 days after implantation into the brain, demonstrating long-term delivery of the protein. In vitro, we demonstrate that PCL with immobilised GDNF (iGDNF) significantly enhances cell viability and neural stem cell/progenitor proliferation compared to conventional 2-dimensional cultureware. Upon implantation, PCL scaffolds including iGDNF enhanced the survival, proliferation, migration, and neurite growth of transplanted cortical cells, whilst suppressing inflammatory reactive astroglia.

Keywords
Polycaprolactone; Scaffold; Neural stem cell; Transplantation; GDNF; PlasticityCNS, central nervous system; CRT, cell replacement therapy; E, embryonic day; ED, ethylene diamine; ELISA, enzyme-linked immunosorbent assay; GDNF, glial-cell derived neurotrop
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Promoting engraftment of transplanted neural stem cells/progenitors using biofunctionalised electrospun scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 36, December 2012, Pages 9188–9197
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us