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The role of integrin-linked kinase/β-catenin pathway in the enhanced MG63 differentiation by micro/nano-textured topography

Paper ID Volume ID Publish Year Pages File Format Full-Text
6840 522 2013 10 PDF Available
Title
The role of integrin-linked kinase/β-catenin pathway in the enhanced MG63 differentiation by micro/nano-textured topography
Abstract

Micro/nano-texturing is a promising approach to produce biomaterials with better tissue integration properties, but the underlying mechanisms are only partially understood. We propose that the integrin-linked kinase (ILK)/β-catenin pathway may play a role in mediating the signals of topographical cues to cells. To confirm the hypothesis, human MG63 osteoblasts are cultured on the micro/nano-textured topographies (MNTs) to assess the cell differentiation in terms of collagen secretion, extracellular matrix mineralization, and osteogenesis-related gene expression. The expression of β-catenin, ILK and integrin β1 and β3 is assayed by real-time polymerase chain reaction and the protein levels of β-catenin, phosphorylated glycogen synthase kinase 3β (p-GSK3β) and ILK are determined by western blot. The ILK silenced MG63 induced by small interfering RNA is cultured on the samples and the cell functions and the levels of β-catenin, GSK3β and p-GSK3β are determined. The results show that the MNTs enhance MG63 differentiation and it is related to the higher expression of integrin β1 and β3 and ILK, which activate the β-catenin signaling by initiating β-catenin expression and inhibiting its degradation by phosphorylating GSK3β. ILK silencing attenuates the β-catenin signaling activation and the enhanced MG63 differentiation by the MNTs. Our results explicitly demonstrate the role of the ILK/β-catenin pathway in mediating the signals from topographical cues to osteoblasts to tailor differentiation and provide new target points for biomaterials modification and biofunctionalization to attain better clinical performance.

Keywords
Micro/nano-textured topography; Osteoblast; Differentiation; Integrin-linked kinase; β-Catenin
First Page Preview
The role of integrin-linked kinase/β-catenin pathway in the enhanced MG63 differentiation by micro/nano-textured topography
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 3, January 2013, Pages 631–640
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering