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A gold nanoparticle platform for the delivery of functional microRNAs into cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
6858 522 2013 10 PDF Available
Title
A gold nanoparticle platform for the delivery of functional microRNAs into cancer cells
Abstract

Lack of affordable technologies for delivering microRNAs and siRNAs into cells on a large scale has hindered our efforts to rapidly parse through hundreds of dysregulated genes/microRNAs in order to identify drivers of complex diseases. The instability and polyanionic nature of naked microRNAs impede efficient cellular uptake and reduce half-life. Viral delivery requires cloning, microRNA mimics/inhibitors require costly modifications, and both require toxic lipofection or electroporation. To address these challenges, we developed a robust method for delivering unmodified microRNAs into cells on cysteamine-functionalized gold nanoparticles (AuNPs). We validated our method in two different tumor models and found that the best formulation of miR1-AuNP10-S-PEG0.5 had the highest payload (10–20 fold higher than lipofection), lowest toxicity (98% of cell viability following treatment), efficient uptake (96% of cells took it), fastest endosomal escape and increased half-lives (at least 5 days) impacting cell proliferation and patterns of target gene expression.

Keywords
miRNA delivery; In vitro delivery; Gold nanoparticles; Cysteamine-funtionalized; Cancer
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A gold nanoparticle platform for the delivery of functional microRNAs into cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 3, January 2013, Pages 807–816
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us