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Tyroserleutide-based gene vector for suppressing VEGF expression in cancer therapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
6932 524 2012 10 PDF Available
Title
Tyroserleutide-based gene vector for suppressing VEGF expression in cancer therapy
Abstract

A small interfering RNA (siRNA) plasmid DNA (pYr-1.1-hU6-EGFP-siVEGF) was constructed and used for suppressing vascular endothelial growth factor (VEGF) expression and inhibiting tumor growth. Then, a (tyrosyl–seryl–leucine)–polyethyleneimine–poly(ethylene glycol) (YSL–PEI–PEG) conjugate was designed and synthesized as a gene carrier for the delivery of pYr-1.1-hU6-EGFP-siVEGF plasmid. The therapeutic peptide YSL was conjugated to PEI to improve the anti-cancer efficiency, and the PEG chain was introduced to reduce the serum protein adsorption and improve the stability of the complex in the systemic circulation. It was found that YSL–PEI–PEG could efficiently condense plasmid DNA when the vector/DNA weight ratio was higher than 2. Compared with PEI 25 kDa, YSL–PEI–PEG exhibited higher transfection efficiency and lower cytotoxicity. More importantly, the results showed that the gene delivery system owned strong ability to inhibit cancer cell proliferation in vitro and tumor growth in vivo. YSL–PEI–PEG has great potential as a gene vector for clinical applications.

Keywords
Gene therapy; YSL; RNA interference; VEGF
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Tyroserleutide-based gene vector for suppressing VEGF expression in cancer therapy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 33, November 2012, Pages 8685–8694
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us