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Super long-term glycemic control in diabetic rats by glucose-sensitive LbL films constructed of supramolecular insulin assembly

Paper ID Volume ID Publish Year Pages File Format Full-Text
6937 524 2012 10 PDF Available
Title
Super long-term glycemic control in diabetic rats by glucose-sensitive LbL films constructed of supramolecular insulin assembly
Abstract

The supramolecular assembly of porcine insulin (P-SIA) is prepared at moderate condition and monitored by Thioflavin T fluorescence to avoid the formation of mature amyloid fibrils with β-sheet rich structure. P-SIA is characterized in terms of structure, morphology and the capability of sustained insulin release in vitro. Then, a glucose-sensitive layer-by-layer (LbL) film is fabricated with star poly[2-(dimethylamino)ethyl methacrylate] (star-PDMAEMA), glucose oxidase (GOD), catalase (CAT) and P-SIA in the form of {(Star-PDMAEMA/P-SIA)2 + (Star-PDMAEMA/CAT)1 + (Star-PDMAEMA/GOD)2}2 + Star-PDMAEMA, in which the CAT is introduced to eliminate the aggregated H2O2 and maintain the activity of GOD in the long release time. Within the scope of the investigation, a single dose administration could provide effective glycemic control in diabetic rats for up to 295 days without hypoglycemia. The striking result is contributed both by the inherent property of P-SIA and the glucose-sensitive regulation capability of the LbL film, for which the mechanism was thoroughly investigated both in vitro and in vivo. These findings are valuable to inspire more researches to combine supramolecular insulin assembly with various functional drug delivery systems, biochemical additives, biomaterials and biomedical devices for diabetic therapy.

Keywords
Diabetes; Drug delivery; Long-term controlled release; Supramolecular insulin assembly; Layer-by-layer assembly
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Super long-term glycemic control in diabetic rats by glucose-sensitive LbL films constructed of supramolecular insulin assembly
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 33, November 2012, Pages 8733–8742
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us