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A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions

Paper ID Volume ID Publish Year Pages File Format Full-Text
6985 527 2012 12 PDF Available
Title
A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions
Abstract

Realizing the potential of human pluripotent stem cell (hPSC)-based therapy requires the development of defined scalable culture systems with efficient expansion, differentiation and isolation protocols. We report an engineered 3D microfiber system that efficiently supports long-term hPSCs self-renewal under chemically defined conditions. The unique feature of this system lies in the application of a 3D ECM-like environment in which cells are embedded, that affords: (i) uniform high cell loading density in individual cell-laden constructs (∼107 cells/ml); (ii) quick recovery of encapsulated cells (<10 min at 37 °C) with excellent preservation of cell viability and 3D multicellular structure; (iii) direct cryopreservation of the encapsulated cells in situ in the microfibers with >17-fold higher cell viability compared to those cultured on Matrigel surface; (iv) long-term hPSC propagation under chemically defined conditions. Four hPSC lines propagated in the microfibrous scaffold for 10 consecutive passages were capable of maintaining an undifferentiated phenotype as demonstrated by the expression of stem cell markers and stable karyotype in vitro and the ability to form derivatives of the three germ layers both in vitro and in vivo. Our 3D microfibrous system has the potential for large-scale cultivation of transplantable hESCs and derivatives for clinical applications.

Keywords
Stem cell; Scaffold; Cell culture; Cell encapsulation; Cell proliferation
First Page Preview
A 3D microfibrous scaffold for long-term human pluripotent stem cell self-renewal under chemically defined conditions
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 8, March 2012, Pages 2419–2430
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering