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MUC-1 aptamer-conjugated dye-doped silica nanoparticles for MCF-7 cells detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
7005 528 2013 11 PDF Available
Title
MUC-1 aptamer-conjugated dye-doped silica nanoparticles for MCF-7 cells detection
Abstract

In this work, we have prepared three types of aptamer-conjugated Rubpy-doped silica nanoparticles for Human breast carcinoma MCF-7 cells labeling. Probe A is prepared through covalent conjugation between amine-labeled MUC-1 aptamer and carboxyl-modified Rubpy-doped NPs (NPs-aptamer). Probe B is prepared based on the interaction between biotin-labeled MUC-1 aptamer and avidin-conjugated Rubpy-doped NPs (NPs-avidin-biotin-aptamer). For Probe C, there is a PEG with flexible long chain as the bridge between avidin and the NPs (NPs-PEG-avidin-biotin-aptamer). In addition, we further investigate the practical number of MUC-1 aptamers on an NP of each probe using hoechst33258 dye. The binding efficiency of MUC-1 aptamer on the three types of probes as follows: Probe A < Probe B < Probe C. In addition, microscopic fluorescence imaging shows that Probe C containing the PEG molecules can be effectively applied for the recognition of MUC-1 protein in human breast carcinoma MCF-7 cells thus demonstrates that the PEG with flexible long chain as the bridge between the aptamer and NP can greatly enhances the freedom of MUC-1 aptamer. Compared with common organic dyes, the dye-doped silica nanoparticles serve as a stable bioprobe because of their facile conjugation with the desirable biomolecules, and have exhibited great potential in bioanalysis.

Keywords
Dye-doped silica nanoparticles; Aptamer; Conjugation; MCF-7 cells
First Page Preview
MUC-1 aptamer-conjugated dye-doped silica nanoparticles for MCF-7 cells detection
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 2, January 2013, Pages 371–381
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering