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Endonuclease-responsive aptamer-functionalized hydrogel coating for sequential catch and release of cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
7014 528 2013 10 PDF Available
Title
Endonuclease-responsive aptamer-functionalized hydrogel coating for sequential catch and release of cancer cells
Abstract

Rare circulating tumor cells are a promising biomarker for the detection, diagnosis, and monitoring of cancer. However, it remains a challenge to develop biomedical devices for specific catch and nondestructive release of circulating tumor cells. The purpose of this study was to explore a unique system for cell catch and release by using aptamer-functionalized hydrogels and restriction endonucleases. The results show that the hydrogel coating was highly resistant to nonspecific cell binding with ∼5–15 cells/mm2 on the hydrogel surface. In contrast, under the same condition, the aptamer-functionalized hydrogel coating could catch target cancer cells with a density over 1000 cells/mm2. When the hydrogel coating was further treated with the restriction endonucleases, the bound cells were released from the hydrogel coating because of the endonuclease-mediated sequence-specific hydrolysis of the aptamer sequences. The release efficiency reached ∼99%. Importantly, ∼98% of the released cells maintained viability. Taken together, this study demonstrates that it is promising to apply endonuclease-responsive aptamer-functionalized hydrogels as a coating material to develop medical devices for specific catch and nondestructive release of rare circulating tumor cells.

Keywords
Hydrogel; Affinity; Interface; Enzyme; Cell adhesion; Biocompatibility
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Endonuclease-responsive aptamer-functionalized hydrogel coating for sequential catch and release of cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 34, Issue 2, January 2013, Pages 460–469
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us