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Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
7130 535 2011 11 PDF Available
Title
Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles
Abstract

Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.

Keywords
Silicon; Microparticle; Hydrophobin; Biocompatibility; Drug release
First Page Preview
Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 34, December 2011, Pages 9089–9099
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering