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Consequences of ineffective decellularization of biologic scaffolds on the host response

Paper ID Volume ID Publish Year Pages File Format Full-Text
7160 537 2012 11 PDF Available
Title
Consequences of ineffective decellularization of biologic scaffolds on the host response
Abstract

Biologic scaffold materials composed of extracellular matrix (ECM) are routinely used for a variety of clinical applications. Despite known variations in tissue remodeling outcomes, quantitative criteria by which decellularization can be assessed were only recently described and as a result, the amount of retained cellular material varies widely among commercial products. The objective of this study was to evaluate the consequences of ineffective decellularization on the host response. Three different methods of decellularization were used to decellularize porcine small intestinal ECM (SIS-ECM). The amount of cell remnants was quantified by the amount and fragmentation of DNA within the scaffold materials. The M1/M2 phenotypic polarization profile of macrophages, activated in response to these ECM scaffolds, was assessed in vitro and in vivo using a rodent model of body wall repair. The results show that, in vitro, more aggressive decellularization is associated with a shift in macrophage phenotype predominance from M1 to M2. While this shift was not quantitatively apparent in vivo, notable differences were found in the distribution of M1 vs. M2 macrophages within the various scaffolds. A clear association between macrophage phenotype and remodeling outcome exists and effective decellularization remains an important component in the processing of ECM-based scaffolds.

Keywords
Extracellular matrix; Macrophage; Decellularization; Immune response; Scaffold
First Page Preview
Consequences of ineffective decellularization of biologic scaffolds on the host response
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 33, Issue 6, February 2012, Pages 1771–1781
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering