fulltext.study @t Gmail

Gadolinium-labeled peptide dendrimers with controlled structures as potential magnetic resonance imaging contrast agents

Paper ID Volume ID Publish Year Pages File Format Full-Text
7200 540 2011 10 PDF Available
Title
Gadolinium-labeled peptide dendrimers with controlled structures as potential magnetic resonance imaging contrast agents
Abstract

Gadolinium (Gd3+) based dendrimers with precise and tunable nanoscopic sizes are excellent candidates as magnetic resonance imaging (MRI) contrast agents. Control of agents’ sensitivity, biosafety and functionality is key to the successful applications. We report the synthesis of Gd(III)-based peptide dendrimers possessing highly controlled and precise structures, and their potential applications as MRI contrast agents. These agents have no obvious cytotoxicity as verified by in vitro studies. One of the dendrimer formulations with mPEG modification showed a 9-fold increase in T1 relaxivity to 39.2 Gd(III) mM−1 s−1 comparing to Gd-DTPA. In vivo studies have shown that the mPEGylated Gd(III)-based dendrimer provided much higher signal intensity enhancement (SI) in mouse kidney, especially at 60 min post-injection, with 54.8% relatively enhanced SI. The accumulations of mPEGylated dendrimer in mouse liver and kidney were confirmed through measurement of gadolinium by inductively coupled plasma atomic emission spectroscopy (ICP-AES). Meanwhile, mPEGylated dendrimer showed much higher Gd(III) concentration in blood with 38 μg Gd(III)/g blood at 1 h post-injection comparing to other dendrimer formulations. These findings provide an attractive alternative strategy to the design of multifunctional gadolinium-based dendrimers with controlled structures, and open up possibilities of using the Gd(III)-based peptide dendrimers as MRI probes.

Keywords
Peptide dendrimer; Gadolinium; MRI; Contrast agent; Blood circulation
First Page Preview
Gadolinium-labeled peptide dendrimers with controlled structures as potential magnetic resonance imaging contrast agents
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 31, November 2011, Pages 7951–7960
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering