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Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization

Paper ID Volume ID Publish Year Pages File Format Full-Text
7202 540 2011 7 PDF Available
Title
Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization
Abstract

An unresolved obstacle in transplantation of islets of Langerhans is the early graft loss caused by thrombotic reactions on the surface of islets after intraportal transplantation. We investigated a versatile method for modifying the surface of islets with liposomes carrying the anticoagulant argatroban using an amphiphilic poly(ethylene glycol)-phospholipid conjugate derivative (PEG-lipid) and DNA hybridization. Argatroban was gradually released from the liposomes on the islets, and antithrombic activity was detected in culture medium. Modified islets retained the ability to control insulin release in response to glucose concentration changes. Although we mainly examined surface modification of islets, this technique may be useful for immobilizing various types of small molecules on cells and tissues and thus may have many applications in cell therapy and regenerative medicine.

Keywords
Islet of Langerhans; Poly(ethylene glycol)-lipid; Argatroban; Liposome; polyDNA hybridization; Surface modification
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Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 31, November 2011, Pages 7971–7977
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us